Dephosphorylation of Phytate by Using the Aspergillus niger Phytase with a High Affinity for Phytate

Dephosphorylation of phytate by using the Aspergillus niger phytase with a high affinity for phytate.

A phytase (EC 3.1.3.8) with a high affinity for phytic acid was found in Aspergillus niger SK-57 and purified to homogeneity in four steps by using ion-exchange chromatography (two types), gel filtration, and chromatofocusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme gave a single stained band at a molecular mass of approximately 60 kDa. The Michaelis con...

Improved phytate phosphorus utilization by Japanese medaka transgenic for the Aspergillus niger phytase gene.

The inefficient digestion of phytate phosphorus by fish has created environmental concerns associated with phosphorus pollution from aquaculture production facilities. To further complicate this situation, phytate is known to chelate minerals and proteins, making them nutritionally unavailable. The enzyme phytase degrades phytate into inorganic phosphorus, which can be directly utilized by fish...

Utilization of Aspergillus niger Phytase Preparation for Hydrolysis of Phytate in Foods

Phytate and phytase: consequences for protein utilisation.

The excretion of large amounts of P in effluent from intensive pig and poultry units is indicative of the poor availability of phytate-bound P in plant-derived feed ingredients. This environmental problem prompted the development and acceptance of microbial phytase feed enzymes for single-stomached animals. Their introduction led to an increasing recognition that phytate may have adverse effect...

Phytate and Phytase in Broilers --a Review

The vegetable feed ingredients used in the preparation of poultry feed contains nearly two thirds of the phosphorus as phytates. The phytates are not only indigestible /unavailable but also are known to interfere with the utilization of other nutrients. Apart from the anti-nutritional effects the phytates bind with other minerals and make them unavialble to the birds. One of the best ways to ov...